|
KMID : 0364820080440040299
|
|
Korean Journal of Microbiology
2008 Volume.44 No. 4 p.299 ~ p.304
|
|
|
Cell Cycle Arrest in Human Monocyte Cell Line by Human Cytomegalovirus
|
|
Jang So-Young
Kim Mi-Suk
Lee Chan-Hee
|
|
|
Abstract
|
|
|
|
Monocytic cells in myeloid lineage are known for latent site of HCMV. Previous studies have suggested that HCMV regulates cell cycle progression in a variety of cells, but studies in monocytic cells are limited. In this study, we attempted to understand cell cycle changes after HCMV infection in the monocytic cell lines. Flow cytometric analyses using propidium iodide revealed that the proportion of G0-G1 phase was increased and the proportion of S phase decreased in HCMV-infected THP-1 cells, but not in HL-60 cells. BrdU-incorporation assay supported that cell proliferation was inhibited in HCMV-infected THP-1 cells by inhibition of de novo DNA synthesis. Western blot analysis revealed that p21, inhibitor of cell cycle progression from G1 phase to S phase, was induced in HCMV-infected THP-1 cells but not in HL-60 cells. Thus, HCMV inhibited cell proliferation by arresting the cell cycle at G0-G1 phase through induction of p21 protein in promocytic THP-1 cells.
|
|
|
KEYWORD
|
|
|
cell cycle, human cytomegalovirus, monocytes
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|